Mouse Anti-FER Recombinant Antibody (clone 5D2C4)
CAT#: VS3-XY700
Clone 5D2C4 is a mouse antibody, which reacts with human FER protein. This antibody is suitable for FER detection by ELISA, WB, IHC, ICC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7641/45_B5_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in lymphoid cells outside reaction center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7641/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/54455_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/54455_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/54455_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/54455_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/54455_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22464/54455_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151422-FER
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Specifications
- Immunogen
- Recombinant fragment of human FER expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human FER
- Species Reactivity
- Human
- Clone
- 5D2C4
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- FER Tyrosine Kinase; Fujinami Poultry Sarcoma/Feline Sarcoma-Related Protein Fer; Protein Phosphatase 1, Regulatory Subunit 74; Feline Encephalitis Virus-Related Kinase FER; Fer (Fps/Fes Related) Tyrosine Kinase; Phosphoprotein NCP94; Proto-Oncogene C-Fer; Tyrosine Kinase 3
- Gene ID
- 2241
- UniProt ID
- P16591
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "FER"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1989MZ | Recombinant Mouse Anti-Human FER Antibody (clone 3469D4b) | WB | Mouse antibody |
| ZG-0339J | Mouse Anti-FER Recombinant Antibody (ZG-0339J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-CJ704 | Mouse Anti-FER Recombinant Antibody (VS3-CJ704) | WB, IHC | Mouse IgG1, κ |
| VS3-WK812 | Mouse Anti-FER Recombinant Antibody (VS3-WK812) | WB, ICC, FC | Mouse IgG2a, κ |
| VS3-FY2609 | Mouse Anti-Fer Recombinant Antibody (VS3-FY2609) | WB, ICC, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1283 | Hi-Affi™ Rabbit Anti-FER Recombinant Antibody (clone DS1283AB) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY829 | Anti-FER Immunohistochemistry Kit | IHC | |
| VS-0525-XY2500 | Anti-Human FER Immunohistochemistry Kit | IHC |
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