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    Mouse Anti-NBN Recombinant Antibody (clone 7E4A2) (CAT#: VS3-XY1135)

    Clone 7E4A2 is a mouse antibody, which reacts with human NBN protein. This antibody is suitable for NBN detection by ELISA, WB, IHC, FC, ICC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/if_selected.jpg
    Figure 2 IHC staining of human testis
    Figure 2 IHC staining of human testis
    (Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferous ducts and Leydig cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_4_6_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_5.jpg
    Figure 7 Cervix
    Figure 7 Cervix
    (Glandular cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_7_3.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3)
    Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104320-NBN

    Specifications

    • Immunogen
    • Recombinant fragment of human NBN (a.a. 467-615) expressed in E. coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2a
    • Specificity
    • Human NBN
    • Species Reactivity
    • Human
    • Clone
    • 7E4A2
    • Applications
    • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry

    Product Property

    • Purification
    • Affinity purified
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).

    Target

    • Alternative Names
    • Nibrin; Nijmegen Breakage Syndrome 1 (Nibrin); Cell Cycle Regulatory Protein P95; NBS1; NBS; P95

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MHH-692-F(E) Recombinant Human Anti-human NBN Antibody Fab Fragment IF, FC, FuncS Fab

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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