Mouse Anti-TBX5 Recombinant Antibody (clone 7B11)
CAT#: VS3-XY1488
Clone 7B11 is a mouse antibody, which reacts with human TBX5 protein. This antibody is suitable for TBX5 detection by ELISA, WB assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1331_B9_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1396_B4_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1345_B9_4_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_4_6.jpg
Normal Tissue
Figure 10 Heart muscle
Cardiomyocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_B_6_6.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HAP1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000089225-TBX5
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Specifications
- Immunogen
- Recombinant fragment of TBX5 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TBX5
- Species Reactivity
- Human
- Clone
- 7B11
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "TBX5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-599 | Human Anti-TBX5 Recombinant Antibody (clone D57); Fab Fragment | IP, IF, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
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| MOB-1904MZ | Recombinant Mouse Anti-Human TBX5 Antibody | WB | Mouse antibody |
| ZG-130R | Mouse Anti-TBX5 Recombinant Antibody (ZG-130R) | WB, ELISA | Mouse IgG |
| VS-0625-FY1481 | Human Anti-TBX5 Recombinant Antibody (clone 2506W1376) | ELISA, Spiked IP, IF | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2252 | Anti-TBX5 Immunohistochemistry Kit | IHC |
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