Human Anti-TBX5 Recombinant Antibody (clone D57); Fab Fragment

Name: Human Anti-TBX5 Recombinant Antibody (clone D57); Fab Fragment
SKU: DrMAB-599
Rating: 5 (1 reviews)

CAT#: DrMAB-599

Recombinant Human Antibody binds selectively to Human TBX5, expressed in E. coli.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1331_B9_2_selected.jpg

Subcellular Location Figure 2 IHC staining of human heart muscle

Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line RT4

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1396_B4_1_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1345_B9_4_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_B_8_5.jpg

Normal Tissue Figure 6 Colon

Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_9_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_8_4.jpg

Normal Tissue Figure 8 Kidney

Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_8_5.jpg

Normal Tissue Figure 9 Testis

Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_4_6.jpg

Normal Tissue Figure 10 Heart muscle

Cardiomyocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_B_6_6.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HAP1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000089225-TBX5

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Specifications

Host Species
Human

Type
Human Fab

Specificity
Human TBX5

Species Reactivity
Human

Clone
D57

Applications
IP, IF, ELISA

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TBX5; T-box 5; HOS; T-box transcription factor TBX5; T-box protein 5

Gene ID
6910

UniProt ID
Q99593

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - DrMAB-599.pdf

MSDS

MSDS - DrMAB-599.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "TBX5"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1904MZ	Recombinant Mouse Anti-Human TBX5 Antibody	WB	Mouse antibody
ZG-130R	Mouse Anti-TBX5 Recombinant Antibody (ZG-130R)	WB, ELISA	Mouse IgG
VS3-XY1488	Mouse Anti-TBX5 Recombinant Antibody (clone 7B11)	ELISA, WB	Mouse IgG1
VS-0625-FY1481	Human Anti-TBX5 Recombinant Antibody (clone 2506W1376)	ELISA, Spiked IP, IF	Human IgG1

CAT	Product Name	Application	Type
VS-0325-XY2252	Anti-TBX5 Immunohistochemistry Kit	IHC

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