Human Anti-TBX5 Recombinant Antibody (clone 2506W1376)
CAT#: VS-0625-FY1481
This recombinant antibody is a human IgG1 antibody, produced via recombinant antibody (rAb) phage display, with validated specificity and affinity for the TBX5 protein under non-denaturing conditions. This versatile antibody is suitable for ELISA, Spiked IP, andIF. The antibody targets an epitope within amino acids 55-239 of the TBX5 protein, providing a reliable tool for detecting and analyzing TBX5 in its native conformation across diverse experimental applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1331_B9_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1396_B4_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64683/1345_B9_4_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_A_4_6.jpg
Normal Tissue
Figure 10 Heart muscle
Cardiomyocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8786/62654_B_6_6.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HAP1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000089225-TBX5
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The antibody reacts with human TBX5.
- Species Reactivity
- Human
- Clone
- 2506W1376
- Applications
- ELISA, Spiked Immunoprecipitation, Immunofluorescence
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (over 6 months). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TBX5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-599 | Human Anti-TBX5 Recombinant Antibody (clone D57); Fab Fragment | IP, IF, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1904MZ | Recombinant Mouse Anti-Human TBX5 Antibody | WB | Mouse antibody |
| ZG-130R | Mouse Anti-TBX5 Recombinant Antibody (ZG-130R) | WB, ELISA | Mouse IgG |
| VS3-XY1488 | Mouse Anti-TBX5 Recombinant Antibody (clone 7B11) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2252 | Anti-TBX5 Immunohistochemistry Kit | IHC |
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