Mouse Anti-α-tubulin (acetyl K40) Monoclonal Antibody (W29J3)
CAT#: VS-1225-XL30
Clone W29J3 is a mouse monoclonal identifying α-tubulin acetylated at Lysine 40. This high-specificity reagent is validated for identifying stabilized microtubule structures within both Western Blot and IHC experimental assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/569_B12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43684/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/563_B12_1_blue_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_4_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167552-TUBA1A
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, SCLC-21H)
Cell lines ordered by descending RNA expression order.
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Specifications
- Host Species
- Mouse
- Antibody Isotype
- IgG1
- Specificity
- α-tubulin (acetyl K40)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:1,000-1:2,000
IHC: 1:50-1:100
Target
REVIEWS AND Q&AS
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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