Rabbit Anti-alpha Tubulin (phospho Y272) Monoclonal Antibody
CAT#: VS-0126-XL137
Designed to identify the phosphorylated Tyrosine 272 residue of alpha Tubulin, this rabbit monoclonal ensures accurate capture. It is an effective primary probe for examining microtubule regulation through WB and ICC/IF.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/569_B12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43684/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/563_B12_1_blue_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, SCLC-21H)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167552-TUBA1A
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- α-Tubulin (phospho Y272)
- Species Reactivity
- Human
- Applications
- WB; ICC/IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Processed via a streamlined affinity-chromatography method designed to maximize antibody yield while ensuring peak analytical clarity.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Target
- Alternative Names
- Tubulin alpha-1A chain; Alpha-tubulin 1; TUBA3; LIS3; B-ALPHA-1
- UniProt ID
- P68363
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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