Mouse Anti-UBB Recombinant Antibody (clone 3C12)
CAT#: VS3-XY1573
Clone 3C12 is a mouse antibody, which reacts with human UBB protein. This antibody is suitable for UBB detection by ELISA, WB, FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41344/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/116924_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170315-UBB/subcellular
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Specifications
- Immunogen
- Recombinant fragment of human UBB expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human UBB
- Species Reactivity
- Human
- Clone
- 3C12
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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