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Mouse Anti-UBB Recombinant Antibody (clone 3C12) (CAT#: VS3-XY1573)

Clone 3C12 is a mouse antibody, which reacts with human UBB protein. This antibody is suitable for UBB detection by ELISA, WB, FC assays.


See more Engineered Antibody products for UBB
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human oral mucosa
Figure 1 IHC staining of human oral mucosa
(Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41344/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/116924_B_9_5.jpg
Figure 3 Colon
Figure 3 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_5.jpg
Figure 6 Testis
Figure 6 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_6_6.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170315-UBB/subcellular

Specifications

  • Immunogen
  • Recombinant fragment of human UBB expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human UBB
  • Species Reactivity
  • Human
  • Clone
  • 3C12
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).

Target

  • Alternative Names
  • Ubiquitin B; Polyubiquitin B; Epididymis Secretory Protein Li 50; Polyubiquitin-B; HEL-S-50

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Fab Fragment Antibody

CAT Product Name Application Type
MOB-1021-F(E) Recombinant Anti-human UBB Antibody Fab Fragment IP, FuncS Fab
MHH-1021-F(E) Recombinant Human Anti-human UBB Antibody Fab Fragment WB, IF, FuncS Fab

scFv Fragment Antibody

CAT Product Name Application Type
MOB-1021-S(P) Recombinant Anti-human UBB Antibody scFv Fragment IF, Neut, FuncS scFv
MHH-1021-S(P) Recombinant Human Anti-human UBB Antibody scFv Fragment ELISA, IF, FuncS scFv

Recombinant Antibody

CAT Product Name Application Type
MHH-1021 Recombinant Human Anti-human UBB Antibody ELISA, IF, FuncS IgG
MOB-1208MZ Recombinant Mouse Anti-Human UBB Antibody (clone GQN2) IHC-P Mouse antibody
MRO-1551-CN Rabbit Anti-UBB Recombinant Antibody (clone CBACN-570) WB, IF, IHC, FC Rabbit IgG
VS3-XY1574 Mouse Anti-UBB Recombinant Antibody (clone 2G3) WB, IHC
VS3-WK831 Mouse Anti-UBB Recombinant Antibody (clone 6G6) WB, IHC Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3746 Rabbit Anti-UBB Recombinant Antibody (clone DS3746AB) WB, IHC Rabbit IgG
MOR-4756 Rabbit Anti-UBB Recombinant Antibody (clone TH270DS) WB, IF, ICC, ChIP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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