Recombinant Human Anti-human UBB Antibody
CAT#: MHH-1021
Recombinant Human Antibody specifically reacts with Human UBB, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-UBB mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-UBB mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41344/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/116924_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170315-UBB/subcellular
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Specifications
- Immunogen
- Human ubiquitin B
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human ubiquitin B
- Species Reactivity
- Human
- Applications
- Used for immunoassaytechniques such as: Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional Study
Product Property
- Purity
- >95%, by SDS-PAGE, under reducing conditions
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1021 | Recombinant Anti-human UBB Antibody | ELISA, WB, Dot, FuncS | IgG |
| MOB-1208MZ | Recombinant Mouse Anti-Human UBB Antibody (clone GQN2) | IHC-P | Mouse antibody |
| MRO-1551-CN | Rabbit Anti-UBB Recombinant Antibody (clone CBACN-570) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY1507 | Recombinant Rabbit Anti-UBB Antibody (clone R06-2H7) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK1579 | Mouse Anti-UBB Recombinant Antibody (clone 5F1) | WB, IF, ICC, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1021-F(E) | Recombinant Anti-human UBB Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-1021-F(E) | Recombinant Human Anti-human UBB Antibody Fab Fragment | WB, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1021-S(P) | Recombinant Anti-human UBB Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-1021-S(P) | Recombinant Human Anti-human UBB Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3746 | Rabbit Anti-UBB Recombinant Antibody (clone DS3746AB) | WB, IHC | Rabbit IgG |
| MOR-4756 | Rabbit Anti-UBB Recombinant Antibody (clone TH270DS) | WB, IF, ICC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1200 | CytoStream™ Rabbit Anti-UBB Recombinant Antibody (VS13-YC1200) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2412 | Anti-UBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7687 | Anti-Human UBB Immunohistochemistry Kit | IHC |
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