Rabbit Anti-14-3-3σ (phospho S186) Antibody
CAT#: VS-0126-XL2214
Target-specific rabbit antibody recognizing 14-3-3σ phosphorylated at Serine 186. Primarily utilized in WB to monitor DNA damage-induced regulation of this cell cycle checkpoint protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11105/if_selected.jpg
Normal Tissue
Figure 2 Skin 1
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11105/ihc_selected.jpg
Normal Tissue
Figure 3 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity:>75% Langerhans cells Staining: High Intensity: Strong Quantity:>75% Melanocytes Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40552/84840_B_9_1.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (A-431, HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175793-SFN
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Specifications
- Host Species
- Rabbit
- Specificity
- 14-3-3σ
- Species Reactivity
- Human; Mouse
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Site-specific antibodies are harvested via phosphopeptide matrices, ensuring clarity through secondary depletion of non-phosphorylated immunogen-binding variants.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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