Rabbit Anti-A-Raf (phospho S299) Antibody
CAT#: VS-0126-XL978
Specific for A-Raf phosphorylated at Serine 299, this rabbit polyclonal antibody monitors regulatory modifications in the MAPK pathway. Validated for sensitive immunological detection in IHC, IF, and ELISA.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_7_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078061-ARAF
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Specifications
- Host Species
- Rabbit
- Specificity
- ARAF
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- IHC; IF; ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Extracted from antiserum via affinity chromatography targeting the epitope-specific immunogen.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- IHC: 1:100-1:300
ELISA: 1:5000
IF: 1:50-1:200
Target
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Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| VS-0525-XY488 | Anti-ARAF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY489 | Anti-Mouse ARAF Immunohistochemistry Kit | IHC |
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| VS-0326-YC263 | Mouse Anti-ARAF Recombinant Antibody (AR263) | IP | Mouse IgG2a |
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