Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1994-CN) (CAT#: MRO-1994-CN)
This antibody is a rabbit polyclonal antibody that is capable of binding to MKI67.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
Figure 4 Colon
(Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
(Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
(Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
Specifications
- Immunogen
- Peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MKI67 (Marker Of Proliferation Ki-67)
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry
- Conjugate
- Unconjugated
Product Property
- Purification
- Peptide affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Protein Phosphatase 1, Regulatory Subunit 105; Antigen KI-67; Antigen Ki67; Proliferation-Related Ki-67 Antigen;
- Gene ID
- 4288
- UniProt ID
- P46013
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2237 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) | ELISA, WB, IHC | IgG |
| MOR-4638 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) | IF, ICC, FC, ChIP | IgG |
| MOR-0054-FY | Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025) | ICC, IHC-P, WB | Rabbit IgG |
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