Recombinant Rabbit Anti-ABCG1 Antibody (clone R06-5B9)
CAT#: VS3-FY7
This product is a recombinant rabbit antibody that recognizes human ABCG1 protein. It can detect a band of approximately 76 kDa (observedd molecular weight: 100 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31470/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows strong positivity in cilia of respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31470/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31471/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31471/65722_B_9_5.jpg
Normal Tissue
Figure 5 Lung
Alveolar cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Macrophages Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31470/65726_A_2_4.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31471/65722_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31470/65726_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31471/65722_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity: 75%-25% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31470/65726_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (hTCEpi, JURKAT, MCF7, MOLT-4, OE19, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160179-ABCG1
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WB
Figure 1. Recombinant Rabbit Anti-ABCG1 Antibody (clone R06-5B9) in WB.
Western blot analysis of ABCG1 in HeLa lysates using ABCG1 Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human ABCG1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ABCG1
- Species Reactivity
- Human
- Clone
- R06-5B9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 76 kDa; Observed MW: 100 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- ATP Binding Cassette Subfamily G Member 1; ATP-Binding Cassette Transporter 8; ATP-Binding Cassette, Sub-Family G (WHITE), Member 1; ABC8; White Protein Homolog (ATP-Binding Cassette Transporter 8); ATP-Binding Cassette Transporter Member 1 Of Subfamily G; ATP-Binding Cassette Sub-Family G Member 1
- Gene ID
- 9619
- UniProt ID
- P45844
- Sequence Similarities
- Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
- Cellular Localization
- Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane
- Post Translation Modifications
- Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.
- Protein Refseq
- NP_004906.3; NP_058198.2; NP_997057.1
- Function
- Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed:17408620, PubMed:24576892).
The lipid efflux is ALB-dependent (PubMed:16702602).
Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (PubMed:17408620).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ABCG1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1521z | Mouse Anti-ABCG1 Recombinant Antibody (clone 26A12) | ELISA | Mouse IgG1 |
| MRO-0017-CN | Rabbit Anti-ABCG1 Recombinant Antibody (clone CBACN-007) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ965 | Rabbit Anti-ABCG1 Recombinant Antibody (VS3-CJ965) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0837MZ | Chicken Anti-ABCG1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0015 | Hi-Affi™ Rabbit Anti-ABCG1 Recombinant Antibody (clone DS15AB) | WB, IHC | Rabbit IgG |
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