Recombinant Rabbit Anti-ACADM Antibody (clone R04-4F0)
CAT#: VS3-FY12
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ACADM protein. It can detect a band of approximately 47 kDa (observedd molecular weight: 47 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6198/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26542/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6198/17437_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26542/56058_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26542/56058_A_8_4.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26542/56058_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6198/17437_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Daudi)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117054-ACADM
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-ACADM Antibody (clone R04-4F0) in WB.
Western blot analysis of ACADM/MCAD in rat muscle lysates using ACADM/MCAD Antibody.
❮
❯
Specifications
- Immunogen
- A synthetic peptide of human ACADM/MCAD
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACADM
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4F0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 47 kDa; Observed MW: 47 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Acyl-CoA Dehydrogenase Medium Chain; Acyl-Coenzyme A Dehydrogenase, C-4 To C-12 Straight Chain; Medium-Chain Acyl-CoA Dehydrogenase; MCAD; Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-CoA Dehydrogenase, C-4 To C-12 Straight Chain; Testicular Tissue Protein Li 7
- Gene ID
- 34
- UniProt ID
- P11310
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY12. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ACADM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0029 | Hi-Affi™ Rabbit Anti-ACADM Recombinant Antibody (clone DS29AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK382 | Rabbit Anti-ACADM Recombinant Antibody (clone JE48-09) | WB, ICC, IHC | Rabbit IgG |
| VS7-HM14 | Mouse Anti-ACADM Recombinant Antibody (clone 6F2G12) | WB, IHC, ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY57 | Anti-ACADM Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: IHC, ELISA, FC, WB, ADCC, FuncS
Application: Neut, FC
Application: WB, IHC, ELISA, FC
Application: SPR, Inhib, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: FC, IHC, Cyt, FuncS
Application: Inhib, FC, WB
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
