Recombinant Mouse Anti-ACTA2 Antibody (clone 5C1H11) (CAT#: VS3-FY28)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• ACTA2

• Clone
• 5C1H11

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry

• Conjugate
• Unconjugated

• Purity
• >95%

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.

• Alternative Names
• Actin, Alpha 2, Smooth Muscle, Aorta; Cell Growth-Inhibiting Gene 46 Protein; ACTSA; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; ACTVS

• Gene ID
• 59

• UniProt ID
• P62736

• Sequence Similarities
• Belongs to the actin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
  Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
  Methylated at His-75 by SETD3.
  (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
  The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

• Protein Refseq
• NP_001135417.1; NP_001307784.1; NP_001604.1

• Function
• Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

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