Recombinant Mouse Anti-AR Antibody (clone 8E10-6G2-7F3)
CAT#: VS3-FY93
This product is a recombinant mouse antibody that recognizes human AR protein. The mAb was purified by affinity chromatography.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4733/1914_G6_27_cr5c8779fe8d0bf_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_6_6.jpg
Normal Tissue
Figure 3 High expression in seminal vesicle.
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_9_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, BJ hTERT+, HAP1, LHCN-M2, RH-30, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169083-AR
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IHC
Figure 1. Recombinant Mouse Anti-AR Antibody (clone 8E10-6G2-7F3) in IHC.
Immunohistochemical analysis of paraffin-embedded prostate carcinoma using an androgen receptor antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthesized peptide derived from human Androgen Receptor(AR)
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human AR
- Species Reactivity
- Human
- Clone
- 8E10-6G2-7F3
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- PBS pH 7.3, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/100-1/500
Target
- Alternative Names
- Androgen Receptor; Dihydrotestosterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 4; NR3C4; DHTR; Spinal And Bulbar Muscular Atrophy; Testicular Feminization; Kennedy Disease; HUMARA
- Gene ID
- 367
- UniProt ID
- P10275
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
- Protein Refseq
- NP_000035.2; NP_001011645.1; NP_001334990.1
- Function
- Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849).
Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024).
Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3:
Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Isoform 4:
Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
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Related Diseases
Prostate Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0226 | Hi-Affi™ Rabbit Anti-AR Recombinant Antibody (clone DS226AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| ZG-0031J | Mouse Anti-AR Recombinant Antibody (ZG-0031J) | IHC-P, IF | Mouse IgG |
| ZG-0032J | Mouse Anti-AR Recombinant Antibody (ZG-0032J) | IHC-P, IF | Mouse IgG |
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| VS-0923-FY12 | Recombinant Mouse Anti-AR Antibody (VS-0923-FY12) | ELISA, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY24 | Rat Anti-NHP AR Recombinant Antibody (clone AN1-15) | WB, IHC, IF | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY143 | Anti-AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY485 | Anti-Mouse AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY484 | Anti-Human AR Immunohistochemistry Kit | IHC | |
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