Recombinant Mouse Anti-AR Antibody (clone 8E10-6G2-7F3) (CAT#: VS3-FY93)

• Immunogen
• Synthesized peptide derived from human Androgen Receptor(AR)

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human AR

• Species Reactivity
• Human

• Clone
• 8E10-6G2-7F3

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• PBS pH 7.3, 50% Glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry: 1/100-1/500

• Alternative Names
• Androgen Receptor; Dihydrotestosterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 4; NR3C4; DHTR; Spinal And Bulbar Muscular Atrophy; Testicular Feminization; Kennedy Disease; HUMARA

• Gene ID
• 367

• UniProt ID
• P10275

• Sequence Similarities
• Belongs to the nuclear hormone receptor family. NR3 subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
  Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
  Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

• Protein Refseq
• NP_000035.2; NP_001011645.1; NP_001334990.1

• Function
• Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849).
  Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024).
  Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
  Isoform 3:
  Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  Isoform 4:
  Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Secondary Antibody

• 2ND-049CQ
• Goat Anti-Mouse IgG2b Antibody, R-PE

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS