Recombinant Rabbit Anti-ARNT Antibody (clone R05-3B2) (CAT#: VS3-FY97)
This product is a recombinant rabbit antibody that recognizes human, mouse, rat and hamster ARNT protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 87 kDa).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1. Recombinant Rabbit Anti-ARNT Antibody (clone R05-3B2) in WB.
Western blot analysis of HIF1 β in Hela, CHO-K1, C6, Ramos lysates using HIF1 β Antibody.
IHC
Figure 2. Recombinant Rabbit Anti-ARNT Antibody (clone R05-3B2) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HIF1 β antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Recombinant protein of human HIF1 beta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Hamster ARNT
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R05-3B2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 87 kDa; Observed MW: 87 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Aryl Hydrocarbon Receptor Nuclear Translocator; Class E Basic Helix-Loop-Helix Protein 2; Dioxin Receptor, Nuclear Translocator; HIF-1-Beta; HIF1-Beta; BHLHe2; Hypoxia-Inducible Factor 1, Beta Subunit
- Gene ID
- 405
- UniProt ID
- P27540
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001184254.1; NP_001272964.1; NP_001272965.1
- Function
- Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0917CT | Mouse Anti-ARNT Recombinant Antibody (clone EML171) | IF, IHC-P, WB | Mouse IgG2a |
| MRO-0735-CN | Rabbit Anti-ARNT Recombinant Antibody (clone CBACN-269) | WB, IHC | Rabbit IgG |
| ZG-0102C | Mouse Anti-ARNT Recombinant Antibody (clone 4C5) | WB, IHC-P, IF | Mouse IgG |
| ZG-0550J | Rabbit Anti-ARNT Recombinant Antibody (clone 2F11) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-QX65 | Mouse Anti-ARNT Recombinant Antibody (clone 7E7-6F6-1G2) | WB, IHC | Mouse IgG1 |
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