Recombinant Rabbit Anti-ARNT Antibody (clone R05-3B2)
CAT#: VS3-FY97
This product is a recombinant rabbit antibody that recognizes human, mouse, rat and hamster ARNT protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 87 kDa).
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-ARNT Antibody (clone R05-3B2) in WB.
Western blot analysis of HIF1 β in Hela, CHO-K1, C6, Ramos lysates using HIF1 β Antibody.
IHC
Figure 2. Recombinant Rabbit Anti-ARNT Antibody (clone R05-3B2) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HIF1 β antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/30_F11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/29_F11_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/31_F11_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_4_6.jpg
Normal Tissue
Figure 9 Placenta
Decidual cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_2_7.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enriched (U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143437-ARNT
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Specifications
- Immunogen
- Recombinant protein of human HIF1 beta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Hamster ARNT
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R05-3B2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 87 kDa; Observed MW: 87 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Aryl Hydrocarbon Receptor Nuclear Translocator; Class E Basic Helix-Loop-Helix Protein 2; Dioxin Receptor, Nuclear Translocator; HIF-1-Beta; HIF1-Beta; BHLHe2; Hypoxia-Inducible Factor 1, Beta Subunit
- Gene ID
- 405
- UniProt ID
- P27540
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001184254.1; NP_001272964.1; NP_001272965.1
- Function
- Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ARNT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-214 | Human Anti-ARNT Recombinant Antibody (clone U39) | IP, IF | Human IgG1 |
| MOB-0917CT | Mouse Anti-ARNT Recombinant Antibody (clone EML171) | IF, IHC-P, WB | Mouse IgG2a |
| MRO-0735-CN | Rabbit Anti-ARNT Recombinant Antibody (clone CBACN-269) | WB, IHC | Rabbit IgG |
| ZG-0102C | Mouse Anti-ARNT Recombinant Antibody (clone 4C5) | WB, IHC-P, IF | Mouse IgG |
| ZG-0550J | Rabbit Anti-ARNT Recombinant Antibody (clone 2F11) | ELISA, WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY173 | Anti-ARNT Immunohistochemistry Kit | IHC | |
| VS-0525-XY530 | Anti-Mouse ARNT Immunohistochemistry Kit | IHC | |
| VS-0525-XY529 | Anti-Human ARNT Immunohistochemistry Kit | IHC | |
| VS-0525-XY531 | Anti-Rat ARNT Immunohistochemistry Kit | IHC |
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