Recombinant Rabbit Anti-ATG3 Antibody (clone R06-9E7)
CAT#: VS3-FY108
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ATG3 protein. It can detect a band of approximately 36 kDa (observedd molecular weight: 40 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37260/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40471/139580_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40471/139580_A_6_6.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40471/139580_B_8_5.jpg
Normal Tissue
Figure 5 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40471/139580_B_6_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40471/139580_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144848-ATG3
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WB
Figure 1. Recombinant Rabbit Anti-ATG3 Antibody (clone R06-9E7) in WB.
Western blot analysis of ATG3 in K562, rat brain, C6, 3T3, Hela lysates using ATG3 Antibody.
IHC
Figure 2. Recombinant Rabbit Anti-ATG3 Antibody (clone R06-9E7) in IHC.
Immunohistochemical analysis of paraffin-embedded human tonsil using ATG3 antibody.
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Specifications
- Immunogen
- A synthetic peptide of human ATG3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ATG3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-9E7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 40 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ATG3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0655MZ | Mouse Anti-ATG3 Recombinant Antibody (clone CBLS-113) | WB | Mouse IgG2b |
| MRO-0108-CN | Rabbit Anti-ATG3 Recombinant Antibody (clone JU00-35) | WB, IHC | Rabbit IgG |
| MRO-1653-CN | Rabbit Anti-ATG3 Polyclonal Antibody (MRO-1653-CN) | WB, IHC | Rabbit IgG |
| VS3-QX72 | Mouse Anti-ATG3 Recombinant Antibody (clone 1377CT239.6.1.12) | WB, IHC | Mouse IgG1 |
| VS7-HM154 | Mouse Anti-ATG3 Recombinant Antibody (clone 2C10A12) | WB, IHC, ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0279 | Hi-Affi™ Rabbit Anti-ATG3 Recombinant Antibody (clone DS279AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY35 | Mouse Anti-NHP ATG3 Recombinant Antibody (clone 7A1D1) | WB, IHC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY586 | Anti-ATG3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY587 | Anti-Mouse ATG3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY588 | Anti-Rat ATG3 Immunohistochemistry Kit | IHC |
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