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Recombinant Rabbit Anti-BCKDK Antibody (clone R01-3G4)

Recombinant Rabbit Anti-BCKDK Antibody (clone R01-3G4) (CAT#: VS3-FY146)

Name: Recombinant Rabbit Anti-BCKDK Antibody (clone R01-3G4)
SKU: VS3-FY146
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat BCKDK protein. It can detect a band of approximately 46 kDa (observedd molecular weight: 46 kDa).

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-BCKDK Antibody (clone R01-3G4) in WB.

Figure 1. Recombinant Rabbit Anti-BCKDK Antibody (clone R01-3G4) in WB.

Western blot analysis of BCKDK in PC-12, Raw264.7 lysates using BCKDK Antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56067/if_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_B_7_5.jpg

Figure 3 Salivary gland
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_B_3_1.jpg

Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_A_9_3.jpg

Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_A_9_4.jpg

Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_A_9_5.jpg

Figure 7 Testis
(Elongated or late spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_A_4_6.jpg

Figure 8 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17995/40360_A_9_8.jpg

RNA Expression

Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103507-BCKDK

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human BCKDK

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat BCKDK

Species Reactivity
Human, Mouse, Rat

Clone
R01-3G4

Applications
Western Blot

Conjugate
Unconjugated

MW
Calculated MW: 46 kDa; Observed MW: 46 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000

Target

Alternative Names
Branched Chain Ketoacid Dehydrogenase Kinase; BCKD-Kinase; EC 2.7.11.4; BCKDHKIN; [3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide]] Kinase, Mitochondrial; 3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide] Kinase, Mitochondrial

Gene ID
10295

UniProt ID
O14874

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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IgG Antibody Products Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOB-0908MZ	Mouse Anti-BCKDK Recombinant Antibody (clone CBLS-175)	ELISA	Mouse IgG1

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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