Recombinant Rabbit Anti-Casp9 Antibody (clone R04-8I4) (CAT#: VS3-FY197)
This product is a recombinant rabbit antibody that recognizes mouse and rat Casp9 protein. It can detect a band of approximately 50 kDa (observedd molecular weight: 50 kDa).
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WB
Figure 1. Recombinant Rabbit Anti-Casp9 Antibody (clone R04-8I4) in WB.
Western blot analysis of Caspase9 in 3T3 lysates using Caspase 9 antibody.
WB
Figure 2. Recombinant Rabbit Anti-Casp9 Antibody (clone R04-8I4) in WB.
Western blot analysis of Caspase9 in C6 lysates using Caspase9 Antibody.
Specifications
- Immunogen
- Recombinant protein of mouse Caspase-9
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse and Rat Casp9
- Species Reactivity
- Mouse, Rat
- Clone
- R04-8I4
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Caspase 9; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; ICE-LAP6; APAF-3; MCH6; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3
- Gene ID
- 842
- UniProt ID
- P55211
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Apoptosome, Cytoplasm, Cytosol, Mitochondrion, Nucleus
- Post Translation Modifications
- Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis.
- Protein Refseq
- NP_001220.2; NP_001264983.1; NP_127463.2
- Function
- Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2084z | Mouse Anti-CASP9 Recombinant Antibody (clone 34A5) | WB, IF, IHC, IP | Mouse IgG |
| VS3-QX159 | Mouse Anti-CASP9 Recombinant Antibody (clone 1D1-F2-E9) | WB | Mouse IgG1 |
| VS3-CJ1044 | Mouse Anti-CASP9 Recombinant Antibody (VS3-CJ1044) | WB, IP | Mouse IgG |
| VS3-CJ1045 | Rabbit Anti-CASP9 Recombinant Antibody (VS3-CJ1045) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS7-HM298 | Mouse Anti-CASP9 Recombinant Antibody (clone 5E8C8) | FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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