Recombinant Mouse Anti-CFB Capture Antibody (clone 1E4)
CAT#: VS3-WK1630
This antibody is a mouse recombinant antibody against human CFB. The sandwich ELISA was established using matched antibody pair 1E4 and 5E1. The clone 1E4 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CFB protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/951/170_F11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/1749_A9_28_cr5804bcaa17056_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_3.jpg
Normal Tissue
Figure 7 Rectum
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_4_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_5.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243649-CFB
❮
❯
Specifications
- Immunogen
- Recombinant human CFB protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human CFB
- Species Reactivity
- Human
- Clone
- 1E4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Complement Factor B; B-Factor, Properdin; Properdin Factor B; C3/C5 Convertase; EC 3.4.21.47; PBF2; BFD; GBG; BF; Glycine-Rich Beta-Glycoprotein; Glycine-Rich Beta Glycoprotein
- Gene ID
- 629
- UniProt ID
- P00751
- Long Name
- Complement Factor B
- Sequence Similarities
- Belongs to the peptidase S1 family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_001701.2
- Function
- Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-WK1630. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CFB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-562-VHH | Recombinant Anti-Human CFB VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-059MZ | Anti-Human CFB Recombinant Antibody (FB-28.4.2) | ELISA, WB | |
| TAB-060MZ | Mouse Anti-CFB Recombinant Antibody (TAB-060MZ) | ELISA | Mouse IgG1 |
| TAB-062MZ | Anti-Human CFB Recombinant Antibody (1F7) | ELISA | |
| TAB-059MZ-S(P) | Anti-Human CFB Recombinant Antibody scFv Fragment (FB-28.4.2) | ELISA, WB | |
| TAB-060MZ-S(P) | Mouse Anti-CFB Recombinant Antibody; scFv Fragment (TAB-060MZ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-061MZ | Human Anti-CFB Recombinant Antibody (TAB-061MZ) | ELISA | Humanized antibody |
| TAB-061MZ-S(P) | Human Anti-CFB Recombinant Antibody; scFv Fragment (TAB-061MZ-S(P)) | ELISA | Humanized scFv |
| TAB-061MZ-F(E) | Human Anti-CFB Recombinant Antibody; Fab Fragment (TAB-061MZ-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0673 | Hi-Affi™ Rabbit Anti-CFB Recombinant Antibody (clone DS673AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0145CQ | Human Anti-CFB Recombinant Antibody (HPAB-0145CQ) | ELISA, Inhib, FC | Humanized IgG |
| HPAB-0146CQ | Mouse Anti-CFB Recombinant Antibody (HPAB-0146CQ) | ELISA, Inhib, FC | Mouse IgG |
| HPAB-0119-YJ | Human Anti-CFB Recombinant Antibody (HPAB-0119-YJ) | FuncS, Inhib, Block | Humanized IgG |
| HPAB-AP040-YC | Human Anti-CFB Recombinant Antibody (HPAB-AP040-YC) | ELISA | Human IgG |
| VS3-WK1631 | Recombinant Mouse Anti-CFB Detection Antibody (clone 5E1) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0145CQ-S(P) | Human Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-0145CQ-S(P)) | ELISA, Inhib, FC | Humanized scFv |
| HPAB-0146CQ-S(P) | Mouse Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-0146CQ-S(P)) | ELISA, Inhib, FC | Mouse scFv |
| HPAB-M0511-YC-S(P) | Human Anti-CFB Recombinant Antibody (clone AAfBb); scFv Fragment | ELISA, Inhib | Humanized scFv |
| HPAB-M0512-YC-S(P) | Mouse Anti-CFB Recombinant Antibody (clone AfBb); scFv Fragment | ELISA, Inhib | Mouse scFv |
| FAMAB-0130WJ-S(P) | Human Anti-CFB Recombinant Antibody scFv Fragment (FAMAB-0130WJ-S(P)) | ELISA, WB, RIA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP040-YC-F(E) | Human Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-AP040-YC-F(E)) | ELISA | Human Fab |
| HPAB-AP041-YC-F(E) | Human Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-AP041-YC-F(E)) | ELISA | Human Fab |
| HPAB-AP277-YC-F(E) | Human Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-AP277-YC-F(E)) | ELISA, Inhib | Chimeric (mouse/human) Fab |
| HPAB-AP278-YC-F(E) | Human Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-AP278-YC-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-AP279-YC-F(E) | Mouse Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-AP279-YC-F(E)) | ELISA, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1392 | Anti-CFB Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Block, Cyt, FuncS, Inhib
Application: ELISA, FC, WB, Block
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: WB, ELISA, Neut, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA
Application: Neut, ELISA, WB, SPR, IF, EM
Application: ELISA, FC
Application: ELISA, Block, WB, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
