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    Recombinant Mouse Anti-CFB Capture Antibody (clone 1E4) (CAT#: VS3-WK1630)

    This antibody is a mouse recombinant antibody against human CFB. The sandwich ELISA was established using matched antibody pair 1E4 and 5E1. The clone 1E4 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CFB protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/951/170_F11_1_selected.jpg
    Figure 2 IF staining of human cell line ASC TERT1
    Figure 2 IF staining of human cell line ASC TERT1
    (Immunofluorescent staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/1749_A9_28_cr5804bcaa17056_selected.jpg
    Figure 3 IHC staining of human prostate
    Figure 3 IHC staining of human prostate
    (Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/ihc_selected.jpg
    Figure 4 IHC staining of human prostate
    Figure 4 IHC staining of human prostate
    (Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/ihc_selected.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_B_8_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_3.jpg
    Figure 7 Rectum
    Figure 7 Rectum
    (Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_4_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_5.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)
    Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243649-CFB

    Specifications

    • Immunogen
    • Recombinant human CFB protein
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2b
    • Specificity
    • Human CFB
    • Species Reactivity
    • Human
    • Clone
    • 1E4
    • Applications
    • ELISA
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein G affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.09% sodium azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • ELISA: 1/1000

    Target

    • Alternative Names
    • Complement Factor B; B-Factor, Properdin; Properdin Factor B; C3/C5 Convertase; EC 3.4.21.47; PBF2; BFD; GBG; BF; Glycine-Rich Beta-Glycoprotein; Glycine-Rich Beta Glycoprotein
    • Long Name
    • Complement Factor B
    • Sequence Similarities
    • Belongs to the peptidase S1 family.
    • Cellular Localization
    • Secreted
    • Function
    • Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

    Recommended Products

    Detection antibody

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    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "CFB"

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    CAT Product Name Application Type
    NAB-562-VHH Recombinant Anti-Human CFB VHH Single Domain Antibody IHC, IP, FC, Neut, FUNC Llama VHH

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