Mouse Anti-CFB Recombinant Antibody (clone 37A3)

Name: Mouse Anti-CFB Recombinant Antibody (clone 37A3)
SKU: MOB-2492z
Rating: 5 (1 reviews)

CAT#: MOB-2492z

This product is a mouse antibody that recognizes human CFB. It can be used in the following applications: WB, ELISA, FCM, IHC, FuncS.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/951/170_F11_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line ASC TERT1

Immunofluorescent staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/1749_A9_28_cr5804bcaa17056_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human prostate

Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human prostate

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/ihc_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_B_8_5.jpg

Normal Tissue Figure 6 Colon

Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_3.jpg

Normal Tissue Figure 7 Rectum

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_4_3.jpg

Normal Tissue Figure 8 Liver

Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_5.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243649-CFB

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Specifications

Immunogen
Human Complement Factor B

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG1

Specificity
Human CFB

Species Reactivity
Human

Clone
37A3

Applications
WB, ELISA, FC, IHC, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry, Functional Study.

Target

Alternative Names
CFB; EC 3.4.21.47; Properdin Factor B; C3/C5 Convertase; CFBD; B-Factor, Properdin; BF; ARMD14; Glycine-Rich Beta-Glycoprotein; CFAB; C3 Proaccelerator; C3 Proactivator; PBF2; GBG; EC 3.4.21

UniProt ID
P00751

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-2492z.pdf

MSDS

MSDS - MOB-2492z.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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