Recombinant Mouse Anti-DDIT3 Antibody (clone 2B1) (CAT#: VS3-FY382)
This product is a recombinant mouse antibody that recognizes human, mouse and rat DDIT3. CHOP mouse Monoclonal Antibody(2B1) detects endogenous levels of DDIT3.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58416/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58416/if_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_4_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_9_8.jpg
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTERT-HME1, SK-MEL-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175197-DDIT3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175197-DDIT3
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- CHOP Mouse Monoclonal Antibody(2B1) detects endogenous levels of DDIT3.
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2B1
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western blotting: 1/1000-1/2000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- DNA Damage Inducible Transcript 3; C/EBP Zeta; Growth Arrest And DNA Damage-Inducible Protein GADD153; CCAAT/Enhancer-Binding Protein Homologous Protein; C/EBP-Homologous Protein 10; CHOP-10; GADD153; CHOP10
- Gene ID
- 1649
- UniProt ID
- P35638
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 2B1"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3990z | Mouse Anti-DDIT3 Recombinant Antibody (clone 20G2) | WB, FC, ICC, IHC | Mouse IgG2a |
| MRO-0466-CN | Rabbit Anti-DDIT3 Recombinant Antibody (clone JM10-31) | WB, IHC, FC | Rabbit IgG |
| MRO-1811-CN | Rabbit Anti-DDIT3 Polyclonal Antibody (MRO-1811-CN) | WB, FC | Rabbit IgG |
| VS3-QX319 | Mouse Anti-DDIT3 Recombinant Antibody (clone 10G2-5H3-6D4) | IHC | Mouse IgG1 |
| VS3-QX320 | Mouse Anti-DDIT3 Recombinant Antibody (clone 7G7-3B2-2F10) | WB, IHC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0951 | Hi-Affi™ Rabbit Anti-DDIT3 Recombinant Antibody (clone DS951AB) | IHC-P, WB, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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