Recombinant Rabbit Anti-DSC3 Antibody (clone R06-4G2)
CAT#: VS3-FY462
This product is a recombinant rabbit antibody that recognizes human DSC3 protein. It can detect a band of approximately 100 kDa (observedd molecular weight: 100 kDa).
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-DSC3 Antibody (clone R06-4G2) in WB.
Western blot analysis of Desmocollin 3 in K562, Hela lysates using Desmocollin 3 Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73937/1556_G10_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37328/673_C6_1_selected.jpg
Normal Tissue
Figure 3 Esophagus
High expression in esophagus
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49265/ihc_selected.jpg
Normal Tissue
Figure 4 Skin 1.
High expression in skin 1
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37328/71216_B_7_1_val_selected.jpg
Normal Tissue
Figure 5 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in corneal layer Staining: High Intensity: Strong Quantity: 75%-25% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37328/71216_B_8_1.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37328/71216_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HaCaT, HBEC3-KT, hTCEpi, hTERT-HME1, REH)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134762-DSC3
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Specifications
- Immunogen
- A synthetic peptide of human Desmocollin 3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DSC3
- Species Reactivity
- Human
- Clone
- R06-4G2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 100 kDa; Observed MW: 100 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DSC3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1579z | Mouse Anti-DSC3 Recombinant Antibody (clone 29F1) | WB, ELISA, IF, IP | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0030MZ | Recombinant Mouse Anti-Human DSC3 Antibody (clone Esc4-U115) | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1052 | Hi-Affi™ Rabbit Anti-DSC3 Recombinant Antibody (clone DS1052AB) | WB, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2090 | Anti-Mouse DSC3 Immunohistochemistry Kit | IHC |
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