Recombinant Rabbit Anti-EEF1A1 Antibody (clone R04-3H4)
CAT#: VS3-FY469
This product is a recombinant rabbit antibody that recognizes human and rat EEF1A1 protein. It can detect a band of approximately 50 kDa (observedd molecular weight: 50 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/779_H8_6_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56990/1144_F4_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/126168_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/126168_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/126168_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/126168_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/126168_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51759/126168_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156508-EEF1A1
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WB
Figure 1. Recombinant Rabbit Anti-EEF1A1 Antibody (clone R04-3H4) in WB.
Western blot analysis of eEF1A1/EFTu in U2OS, C6 lysates using eEF1A1/EFTu Antibody.
IHC
Figure 2. Recombinant Rabbit Anti-EEF1A1 Antibody (clone R04-3H4) in IHC.
Immunohistochemical analysis of paraffin-embedded human tonsil using eEF1A1/EFTu antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human eEF1A1/EF-Tu
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat EEF1A1
- Species Reactivity
- Human, Rat
- Clone
- R04-3H4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Eukaryotic Translation Elongation Factor 1 Alpha 1; Leukocyte Receptor Cluster (LRC) Member 7; Leukocyte Receptor Cluster Member 7; Eukaryotic Elongation Factor 1 A-1; Elongation Factor Tu; EF-1-Alpha-1; EEF1A-1; EEF1A; LENG7; EF-Tu; EF1A; Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14; Glucocorticoid Receptor AF-1 Specific Elongation Factor; Translation Elongation Factor 1 Alpha 1-Like 14
- Gene ID
- 1915
- UniProt ID
- P68104
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EEF1A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2636z | Mouse Anti-EEF1A1 Recombinant Antibody (clone 6H2) | WB, ELISA, FC, ICC, IF | Mouse IgG2a, κ |
| MRO-0518-CN | Rabbit Anti-EEF1A1 Recombinant Antibody (clone JB44-13) | WB, IF, IHC, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1124-YJ5 | Human Anti-EEF1A1 Recombinant Autoantibody (clone ANA15) | ELISA, IF, SPR | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY729 | Anti-EEF1A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2161 | Anti-Human EEF1A1 Immunohistochemistry Kit | IHC |
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