Recombinant Rabbit Anti-GAP43 Antibody (clone R01-4F6)
CAT#: VS3-FY577
This product is a recombinant rabbit antibody that recognizes GAP43. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-4F6 is specific for human, mouse, rat GAP43. The antigen is synthetic peptide of human gap43.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13392/1510_B4_2_selected.jpg
Normal Tissue
Figure 2 Colon
Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15600/35117_A_8_3.jpg
Normal Tissue
Figure 3 High expression in cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15600/35117_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172020-GAP43
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WB
Figure 1. Recombinant Rabbit Anti-GAP43 Antibody (clone R01-4F6) in WB.
Western Blot analysis of GAP43 in rat brain lysates using a GAP43 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human GAP43.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat GAP43
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-4F6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 25 kDa; Observed MW: 46 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Growth Associated Protein 43; Axonal Membrane Protein GAP-43; Neural Phosphoprotein B-50; Neuron Growth-Associated Protein 43; Nerve Growth-Related Peptide GAP43; Calmodulin-Binding Protein P-57
- Gene ID
- 2596
- UniProt ID
- P17677
- Sequence Similarities
- Belongs to the neuromodulin family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse
- Post Translation Modifications
- Phosphorylated (By similarity).
Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).
Palmitoylated by ZDHHC3 (By similarity).
Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction (PubMed:14978216).
Deacylated by LYPLA2 (PubMed:21152083).
- Protein Refseq
- NP_001123536.1; NP_002036.1
- Function
- This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GAP43"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0698MZ | Chicken Anti-Gap43 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1397 | Hi-Affi™ Rabbit Anti-GAP43 Recombinant Antibody (clone DS1397AB) | WB, IP, ICC, FC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0629-CN | Rabbit Anti-GAP43 Recombinant Antibody (clone CBACN-234) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1882-CN | Rabbit Anti-GAP43 Polyclonal Antibody (MRO-1882-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK1360 | Mouse Anti-GAP43 Recombinant Antibody (VS3-WK1360) | WB, IF, IHC-P | Mouse IgG |
| VS3-WK1428 | Mouse Anti-GAP43 Antibody (clone Mix-mA) | WB | Mouse IgG1 |
| VS4-WK188 | Mouse Anti-GAP43 Recombinant Antibody (VS4-WK188) | ELISA, WB, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC684 | AbPlus™ Anti-GAP43 Magnetic Beads (VS-0724-YC684) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2725 | Anti-GAP43 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2726 | Anti-Mouse GAP43 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2727 | Anti-Rat GAP43 Immunohistochemistry Kit | IHC |
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