Recombinant Mouse Anti-GATA3 Antibody (clone 5H7C7)
CAT#: VS3-FY580
This product is a recombinant mouse antibody that recognizes GATA3. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry. The clone 5H7C7 is specific for GATA3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29730/295_B8_1_selected.jpg
Normal Tissue
Figure 2 High expression in placenta.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_1_7_val_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_A_7_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16217/140542_B_3_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO, MCF7, RT4, SH-SY5Y, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107485-GATA3
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Clone
- 5H7C7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry.
Target
- Alternative Names
- GATA Binding Protein 3; GATA-Binding Factor 3; Trans-Acting T-Cell-Specific Transcription Factor GATA-3; GATA-Binding Protein 3; HDRS; HDR
- Gene ID
- 2625
- UniProt ID
- P23771
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001002295.1; NP_002042.1
- Function
- Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity).
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GATA3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2040MZ | Recombinant Mouse Anti-Human GATA3 Antibody (clone 1A12-1D9) | ELISA, WB | Mouse antibody |
| ZG-0681J | Rabbit Anti-GATA3 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Rabbit IgG |
| VS7-HM994 | Mouse Anti-GATA3 Recombinant Antibody (clone 2E6H5) | FCM | Mouse IgG1 |
| VS7-HM995 | Mouse Anti-GATA3 Recombinant Antibody (clone CBL313HM) | WB, ELISA | Mouse IgG |
| VS7-HM996 | Mouse Anti-GATA3 Recombinant Antibody (clone CBL314HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2742 | Anti-GATA3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2743 | Anti-Mouse GATA3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2744 | Anti-Rat GATA3 Immunohistochemistry Kit | IHC |
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