Recombinant Mouse Anti-GFAP Antibody (clone 2A9-1E3-5G9)
CAT#: VS3-FY587
This product is a recombinant mouse antibody that recognizes GFAP. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 2A9-1E3-5G9 is specific for human GFAP. The antigen is synthetic peptide derived from human glial fibrillary acidic protein (gfap).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
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IHC-P
Figure 1. Recombinant Mouse Anti-GFAP Antibody (clone 2A9-1E3-5G9) in IHC-P.
Immunohistochemical analysis of paraffin-embedded brain using GFAP antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC-P
Figure 2. Recombinant Mouse Anti-GFAP Antibody (clone 2A9-1E3-5G9) in IHC-P.
Immunohistochemical analysis of paraffin-embedded schwannoma using GFAP antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human glial fibrillary acidic protein (GFAP).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human GFAP
- Species Reactivity
- Human
- Clone
- 2A9-1E3-5G9
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD
- Gene ID
- 2670
- UniProt ID
- P14136
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament
- Post Translation Modifications
- Phosphorylated by PKN1.
- Protein Refseq
- NP_001124491.1; NP_001229305.1; NP_002046.1
- Function
- GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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