Recombinant Rabbit Anti-Mouse GFAP Capture Antibody (clone R36-2N-1) (CAT#: VS3-WK1844)
This antibody is a rabbit recombinant antibody against mouse GFAP. It was developed by immunization of rabbit with recombinant mouse GFAP protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R36-2N-1 and R81-7S-4. The clone R36-2N-1 can be used as a capture antibody in ELISA analysis to identify mouse GFAP.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
Specifications
- Immunogen
- Recombinant mouse GFAP protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse GFAP
- Species Reactivity
- Mouse
- Clone
- R36-2N-1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD
- Gene ID
- 14580
- UniProt ID
- P03995
- Long Name
- Glial fibrillary acidic protein
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament
- Post Translation Modifications
- Phosphorylated by PKN1.
- Protein Refseq
- NP_001124491.1; NP_001229305.1; NP_002046.1
- Function
- GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1288z | Mouse Anti-GFAP Recombinant Antibody (clone 34C2) | WB, ELISA, ICC, IF, IHC | Mouse IgG2b |
| HPAB-0197-WJ | Mouse Anti-GFAP Recombinant Antibody (clone 5C8) | ELISA, IHC | Mouse IgG1 |
| ZG-0231U | Mouse Anti-GFAP Recombinant Antibody (clone 1C91F1) | ELISA, WB, IHC, IF, FC | Mouse IgG2b |
| VS3-CJ646 | Mouse Anti-GFAP Recombinant Antibody (VS3-CJ646) | IHC | Mouse IgG1 |
| VS3-CJ647 | Rabbit Anti-GFAP Recombinant Antibody (VS3-CJ647) | WB, IHC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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