Recombinant Rabbit Anti-H2AFX (phospho Ser139) Antibody (clone R09-4A7)
CAT#: VS3-FY640
This product is a recombinant rabbit antibody that recognizes H2AFX. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R09-4A7 is specific for human, mouse, rat H2AFX. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser139 of human γ h2a.x.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-H2AFX (phospho Ser139) Antibody (clone R09-4A7) in WB.
Western Blot analysis of Gamma H2A.X (Phospho-Ser139) in HepG2 lysates using Phospho-Histone H2A.X (Ser139) Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg
Normal Tissue
Figure 5 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/ihc_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg
Normal Tissue
Figure 9 Kidney
Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg
Normal Tissue
Figure 11 Skin
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg
Normal Tissue
Figure 12 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AX
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser139 of human γ H2A.X.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat H2AFX
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-4A7
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 15 kDa; Observed MW: 15 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "H2AX"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0703J | Rabbit Anti-H2AX Recombinant Antibody (clone 1F10) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ688 | Rabbit Anti-H2AX Recombinant Antibody (VS3-CJ688) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ689 | Rabbit Anti-H2A.X (phospho S139) Recombinant Antibody (VS3-CJ689) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY638 | Recombinant Rabbit Anti-H2AFX Antibody (clone R02-3C8) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY639 | Recombinant Rabbit Anti-H2AFX Antibody (clone R02-5E6) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY984 | Anti-H2AX Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3017 | Anti-Human H2AX Immunohistochemistry Kit | IHC |
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