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H2AX
Recombinant Rabbit Anti-H2AFX Antibody (clone R02-5E6)

Recombinant Rabbit Anti-H2AFX Antibody (clone R02-5E6) (CAT#: VS3-FY639)

Name: Recombinant Rabbit Anti-H2AFX Antibody (clone R02-5E6)
SKU: VS3-FY639
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes H2AFX. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-5E6 is specific for human, mouse, rat H2AFX. The antigen is synthetic peptide of human histone h2a.x.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-H2AFX Antibody (clone R02-5E6) in WB.

Figure 1. Recombinant Rabbit Anti-H2AFX Antibody (clone R02-5E6) in WB.

Western Blot analysis of Histone H2A.X in Hela, Ramos lysates using Histone H2A.X Antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg

Normal Tissue

Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/ihc_selected.jpg

Figure 4 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg

Figure 5 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/ihc_selected.jpg

Figure 6 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg

Figure 7 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg

Figure 8 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg

Figure 9 Kidney
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg

Figure 10 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg

Figure 11 Skin
(Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg

Figure 12 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg

RNA Expression

Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AX

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human histone H2A.X.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat H2AFX

Species Reactivity
Human, Mouse, Rat

Clone
R02-5E6

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 15 kDa; Observed MW: 15 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
H2A Histone Family Member X; Histone H2A.X; H2A/X; H2AX; H2A Histone Family, Member X; H2AX Histone; Histone H2AX; H2A.X

Gene ID
3014

UniProt ID
P16104

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products

CAT	Product Name	Application	Type
ZG-0703J	Rabbit Anti-H2AX Recombinant Antibody (clone 1F10)	ELISA, WB	Rabbit IgG
VS3-CJ688	Rabbit Anti-H2AX Recombinant Antibody (VS3-CJ688)	WB, ICC, IHC, IP, FC	Rabbit IgG
VS3-CJ689	Rabbit Anti-H2A.X (phospho S139) Recombinant Antibody (VS3-CJ689)	WB, IHC	Rabbit IgG
VS3-FY638	Recombinant Rabbit Anti-H2AFX Antibody (clone R02-3C8)	WB, IHC-Fr, IHC-P, ICC, IF, IP	Rabbit IgG
VS3-FY640	Recombinant Rabbit Anti-H2AFX (phospho Ser139) Antibody (clone R09-4A7)	WB, IP	Rabbit IgG

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