Recombinant Rabbit Anti-H3F3A Antibody (clone R04-5J3) (CAT#: VS3-FY644)

This product is a recombinant rabbit antibody that recognizes H3F3A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R04-5J3 is specific for human, mouse, rat H3F3A. The antigen is synthetic peptide of human histone h3.3.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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We require custom production
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1. Recombinant Rabbit Anti-H3F3A Antibody (clone R04-5J3) in WB.

Figure 1. Recombinant Rabbit Anti-H3F3A Antibody (clone R04-5J3) in WB.

Western Blot analysis of histone H3.3 in K562, C6, 3T3 lysates using a histone H3.3 antibody.


Specifications

  • Immunogen
  • Synthetic peptide of human histone H3.3.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat H3F3A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-5J3
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 15 kDa; Observed MW: 15 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100

Target

  • Alternative Names
  • H3 Histone Family Member 3A; H3 Histone, Family 3A; H3.3A; H3F3; Histone H3.3; H3F3A H3F3B; H3.3B

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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ZG-0704J Rabbit Anti-H3-3A Recombinant Antibody (clone 28H4) ELISA, WB, ICC, FC Rabbit IgG
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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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