Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0) (CAT#: VS3-FY648)
This product is a recombinant rabbit antibody that recognizes HIST1H3A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation. The clone R08-4A0 is specific for human HIST1H3A. The antigen is a synthetic acetyl peptide corresponding to residues surrounding lys27 of human histone h3.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using an antibody to histone H3 (acetylLys27). High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- A synthetic acetyl peptide corresponding to residues surrounding Lys27 of human histone H3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HIST1H3A
- Species Reactivity
- Human
- Clone
- R08-4A0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 15 kDa; Observed MW: 15 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Chromatin Immunoprecipitation: 1/20
Target
- Alternative Names
- Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3/A; H3FA; HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J; Histone H3.1; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I
- Gene ID
- 8350
- UniProt ID
- P68431
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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