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Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0)

Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0) (CAT#: VS3-FY648)

Name: Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0)
SKU: VS3-FY648
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes HIST1H3A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation. The clone R08-4A0 is specific for human HIST1H3A. The antigen is a synthetic acetyl peptide corresponding to residues surrounding lys27 of human histone h3.

Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC-P

Figure 1. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0) in IHC-P.

Figure 1. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using an antibody to histone H3 (acetylLys27). High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Normal Tissue

Figure 1 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_B_7_5.jpg

Figure 2 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_3.jpg

Figure 3 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_7_4.jpg

Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_9_5.jpg

Figure 5 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_4_6.jpg

Figure 6 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_8.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000275714-H3C1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic acetyl peptide corresponding to residues surrounding Lys27 of human histone H3.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human HIST1H3A

Species Reactivity
Human

Clone
R08-4A0

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 15 kDa; Observed MW: 15 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Chromatin Immunoprecipitation: 1/20

Target

Alternative Names
Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3/A; H3FA; HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J; Histone H3.1; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I

Gene ID
8350

UniProt ID
P68431

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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VS3-WK1412	Mouse Anti-H3C1 Antibody (clone Mix-mA)	WB, IHC, IF	Mouse IgG1
VS3-WK2176	Rabbit Anti-H3C1 (pS10) Recombinant Antibody (clone BP6092)	IHC-P	Rabbit IgG
VS3-WK2196	Rabbit Anti-H3C1 (acetyl K14) Recombinant Antibody (clone R06-2F5)	WB, ICC, IF, IP	Rabbit IgG
VS3-WK2197	Rabbit Anti-H3C1 (acetyl K27) Recombinant Antibody (clone R49-6E-4)	WB, IHC-P, ICC, IF	Rabbit IgG
VS3-WK2199	Rabbit Anti-H3C1 (acetyl K56) Recombinant Antibody (clone R02-5J8)	WB, IHC-P, ICC, IF	Rabbit IgG

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