Recombinant Rabbit Anti-HK1 Antibody (clone R02-4A8)
CAT#: VS3-FY674
This product is a recombinant rabbit antibody that recognizes HK1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R02-4A8 is specific for human, mouse, rat HK1. The antigen is synthetic peptide of human hexokinase 1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/946_E10_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7043/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/ihc_selected.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_6_6.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156515-HK1
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WB
Figure 1. Recombinant Rabbit Anti-HK1 Antibody (clone R02-4A8) in WB.
Western Blot analysis of Hexokinase I in K562, Rat Brain, 3T3 lysates using Hexokinase I Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human hexokinase 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat HK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-4A8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 102 kDa; Observed MW: 102 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3500z | Mouse Anti-HK1 Recombinant Antibody (clone 18G4) | WB, ELISA, ICC, IF | Mouse IgG2a, κ |
| HPAB-0058-LSX | Human Anti-HK1 Recombinant Antibody (HPAB-0058-LSX) | ELISA, Inhib, FuncS | Humanized IgG |
| MRO-0734-CN | Rabbit Anti-HK1 Recombinant Antibody (clone CBACN-268) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK796 | Rabbit Anti-HK1 Recombinant Antibody (clone ST47-05) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| VS4-WK219 | Mouse Anti-HK1 Recombinant Antibody (VS4-WK219) | ELISA, WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1614 | Rabbit Anti-HK1 Recombinant Antibody (clone DS1614AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0058-LSX-S(P) | Human Anti-HK1 Recombinant Antibody; scFv Fragment (HPAB-0058-LSX-S(P)) | ELISA | Humanized scFv |
| HPAB-0059-LSX-S(P) | Human Anti-HK1 Recombinant Antibody; scFv Fragment (HPAB-0059-LSX-S(P)) | ELISA | Humanized scFv |
| HPAB-0060-LSX-S(P) | Human Anti-HK1 Recombinant Antibody; scFv Fragment (HPAB-0060-LSX-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0058-LSX-F(E) | Human Anti-HK1 Recombinant Antibody; Fab Fragment (HPAB-0058-LSX-F(E)) | ELISA | Humanized Fab |
| HPAB-0059-LSX-F(E) | Human Anti-HK1 Recombinant Antibody; Fab Fragment (HPAB-0059-LSX-F(E)) | ELISA | Humanized Fab |
| HPAB-0060-LSX-F(E) | Human Anti-HK1 Recombinant Antibody; Fab Fragment (HPAB-0060-LSX-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3159 | Anti-HK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3160 | Anti-Mouse HK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3161 | Anti-Rat HK1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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