Recombinant Rabbit Anti-HNRNPK Antibody (clone R07-0A0) (CAT#: VS3-FY693)
This product is a recombinant rabbit antibody that recognizes HNRNPK. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R07-0A0 is specific for human, mouse, rat, Hamster HNRNPK. The antigen is synthetic peptide of human hnrnp k.
Specific Inquiry
WB
Figure 1. Recombinant Rabbit Anti-HNRNPK Antibody (clone R07-0A0) in WB.
Western Blot analysis of hnRNP K in C6, CHO-K1, Hela lysates using hnRNP K Antibody.
Specifications
- Immunogen
- Synthetic peptide of human hnRNP K.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster HNRNPK
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R07-0A0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 58 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Heterogeneous Nuclear Ribonucleoprotein K; Transformation Upregulated Nuclear Protein; HNRPK; TUNP; Transformation Up-Regulated Nuclear Protein; DC-Stretch Binding Protein; HnRNP K; AUKS; CSBP
- Gene ID
- 3190
- UniProt ID
- P61978
- Cellular Localization
- Cell junction, Cell projection, Cytoplasm, Nucleus, Spliceosome
- Post Translation Modifications
- Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.
Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001305115.1; NP_001305116.1; NP_001305117.1
- Function
- One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity).
As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed:33174841).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3722z | Mouse Anti-HNRNPK Recombinant Antibody (clone 38G5) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| ZG-0828F | Mouse Anti-HNRNPK Antibody (clone F45 P9 C7) | WB, IHC | Mouse IgG1 |
| ZG-0742J | Rabbit Anti-HNRNPK Recombinant Antibody (clone 3G7) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-CJ1083 | Rabbit Anti-HNRNPK Recombinant Antibody (VS3-CJ1083) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS-0923-FY243 | Recombinant Mouse Anti-HNRNPK Antibody (VS-0923-FY243) | ELISA, WB | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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