Recombinant Rabbit Anti-ITGB5 Antibody (clone R04-0G6)
CAT#: VS3-FY778
This product is a recombinant rabbit antibody that recognizes ITGB5. This antibody has been reported for use in Western Blot. The clone R04-0G6 is specific for human, Hamster, rat ITGB5. The antigen is human integrin β5 recombinant protein.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-ITGB5 Antibody (clone R04-0G6) in WB.
Western Blot analysis of integrin beta 5 in C6, CHO-K1, Hela lysates using integrin beta 5 antibody.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22050/612_A10_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1820/7522_A_1_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22050/50254_A_8_2_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1820/7522_B_8_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1820/7522_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1820/7522_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1820/7522_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, hTERT-RPE1, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000082781-ITGB5
❮
❯
Specifications
- Immunogen
- Human integrin β5 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat ITGB5
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R04-0G6
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 88 kDa; Observed MW: 88 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY778. Click the button above to contact us or submit your feedback about this product.
Clear Bands In Western Blot
We employed this recombinant rabbit antibody to detect Integrin beta 5 in our hamster and rat cell lines. The clone R04-0G6 recognized the target ITGB5 specifically, producing a clean band at the expected molecular weight of approximately 88 kDa. The affinity purification really shows in the low background we observed on the blots.
High Specificity For Integrin
Our lab needed a high-quality antibody for studying cell adhesion molecules, specifically human integrin beta 5. This product delivered excellent results in our Western Blot assays. The recombinant production ensures that we get the same high performance with every lot, which is crucial for the continuity of our long-term signaling research.
Q&As
-
What is the recommended dilution for Western Blot analysis?
A: For Western Blot applications, we recommend a starting dilution range of 1/500 to 1/1000. However, optimal conditions can vary depending on the sample type and expression levels, so we suggest titrating the antibody in your specific experimental setup to achieve the best results.
-
What is the antigen used for the production of this clone?
A: The clone R04-0G6 was raised against a human integrin beta 5 recombinant protein. It specifically recognizes ITGB5 and has been shown to react with Human, Hamster, and Rat species. This makes it a valuable tool for cross-species research involving this beta subunit of the integrin family.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# VS3-FY778, RRID: AB_3714411)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ITGB5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2480z | Mouse Anti-ITGB5 Recombinant Antibody (clone 36A4) | ELISA, FC, ICC, IHC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2026MZ | Recombinant Mouse Anti-Human ITGB5 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR161 | Mouse Anti-ITGB5 Recombinant Antibody (clone 2B5A7) | ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR162 | Mouse Anti-ITGB5 Recombinant Antibody (clone 1A4H1) | FC | Mouse IgG1 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: FC, IHC, FuncS, Inhib, Cyt
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
