Recombinant Rabbit Anti-JUNB Antibody (clone R01-9B7)
CAT#: VS3-FY787
This product is a recombinant rabbit antibody that recognizes JUNB. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-9B7 is specific for human, mouse, rat JUNB . The antigen is human junb synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19149/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19149/43349_A_5_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4464/15174_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4464/15174_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19149/43349_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4464/15174_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19149/43349_A_4_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4464/15174_B_6_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19149/43349_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171223-JUNB
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WB
Figure 1. Recombinant Rabbit Anti-JUNB Antibody (clone R01-9B7) in WB.
Western Blot analysis of JunB in K562, C6, 3T3, Hela lysates using JunB Antibody.
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Specifications
- Immunogen
- Human JunB synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat JUNB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-9B7
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 42,43 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "JUNB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1430MZ | Recombinant Mouse Anti-Human JUNB Antibody (clone 305D5b) | WB | Mouse antibody |
| MRO-0887-CN | Recombinant Rabbit Anti-JUNB Monoclonal Antibody (CBACN-333) | WB, IF, IP | Rabbit IgG |
| ZG-0042U | Rabbit Anti-JUNB Recombinant Antibody (clone 5H4) | ELISA, IHC, IF | Rabbit IgG |
| VS3-CJ710 | Rabbit Anti-JUNB Recombinant Antibody (VS3-CJ710) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1903 | Hi-Affi™ Recombinant Rabbit Anti-JUNB Monoclonal Antibody (DS1903AB) | WB, ICC, IP | IgG |
| MOR-4504 | Hi-Affi™ Recombinant Rabbit Anti-JUNB Monoclonal Antibody (TH12DS) | WB, IF, ICC, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC223 | AbPlus™ Anti-Junb Magnetic Beads (VS-0724-YC223) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3705 | Anti-JUNB Immunohistochemistry Kit | IHC |
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