Recombinant Rabbit Anti-KIFC1 Antibody (clone R03-3E4)
CAT#: VS3-FY794
This product is a recombinant rabbit antibody that recognizes KIFC1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R03-3E4 is specific for human, mouse KIFC1. The antigen is synthetic peptide of human kifc1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55997/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62674/146969_A_6_8_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62674/146969_A_8_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62674/146969_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000237649-KIFC1
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WB
Figure 1. Recombinant Rabbit Anti-KIFC1 Antibody (clone R03-3E4) in WB.
Western Blot analysis of KIFC1 in K562, 3T3, Hela lysates using KIFC1 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-KIFC1 Antibody (clone R03-3E4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using KIFC1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human KIFC1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse KIFC1
- Species Reactivity
- Human, Mouse
- Clone
- R03-3E4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 74 kDa; Observed MW: 74 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KIFC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2463MZ | Recombinant Mouse Anti-Human KIFC1 Antibody (clone 3C0) | ICC, IF, IHC-P, WB, sELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1943 | Hi-Affi™ Recombinant Rabbit Anti-KIFC1 Monoclonal Antibody (DS1943AB) | WB, ICC, IF, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3782 | Anti-KIFC1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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