Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) (CAT#: VS3-FY817)

This product is a recombinant rabbit antibody that recognizes KRT16. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R02-5F8 is specific for human KRT16. The antigen is recombinant protein of human cytokeratin 16.


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WB

Figure 1. Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) in WB.

Figure 1. Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) in WB.

Western Blot analysis of cytokeratin 16 in HeLa lysates using a cytokeratin 16 antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) in IHC-P.

Figure 2. Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human tonsil using an antibody to cytokeratin 16. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.


Specifications

  • Immunogen
  • Recombinant protein of human cytokeratin 16.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human KRT16
  • Species Reactivity
  • Human
  • Clone
  • R02-5F8
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 51 kDa; Observed MW: 51 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100

Target

  • Alternative Names
  • Keratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin 16, Type I; KRT16A; K16; Keratin, Type I Cytoskeletal 16; Cytokeratin 16; Cytokeratin-16
  • Sequence Similarities
  • Belongs to the intermediate filament family.
  • Cellular Localization
  • Intermediate filament, Keratin
  • Function
  • Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "KRT16"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1980 Hi-Affi™ Recombinant Rabbit Anti-KRT16 Monoclonal Antibody (DS1980AB) IHC-P, IHC-Fr IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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