Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8)
CAT#: VS3-FY817
This product is a recombinant rabbit antibody that recognizes KRT16. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R02-5F8 is specific for human KRT16. The antigen is recombinant protein of human cytokeratin 16.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows moderate cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/136/ihc_selected.jpg
Normal Tissue
Figure 2 Tonsil
Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/136/3363_A_4_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186832-KRT16
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WB
Figure 1. Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) in WB.
Western Blot analysis of cytokeratin 16 in HeLa lysates using a cytokeratin 16 antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-KRT16 Antibody (clone R02-5F8) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using an antibody to cytokeratin 16. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Recombinant protein of human cytokeratin 16.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KRT16
- Species Reactivity
- Human
- Clone
- R02-5F8
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 51 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Keratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin 16, Type I; KRT16A; K16; Keratin, Type I Cytoskeletal 16; Cytokeratin 16; Cytokeratin-16
- Gene ID
- 3868
- UniProt ID
- P08779
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Keratin
- Protein Refseq
- NP_005548.2
- Function
- Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KRT16"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1980 | Hi-Affi™ Recombinant Rabbit Anti-KRT16 Monoclonal Antibody (DS1980AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0237J | Mouse Anti-KRT16 Recombinant Antibody (ZG-0237J) | IHC-P, IF | Mouse IgG |
| ZG-0238J | Mouse Anti-KRT16 Recombinant Antibody (ZG-0238J) | IHC-P, IF | Mouse IgG |
| ZG-1044F | Mouse Anti-KRT16 Recombinant Antibody (clone LL025) | IHC-Fr, IHC-P, WB | Mouse IgG1 |
| VS3-CJ524 | Mouse Anti-KRT16 Recombinant Antibody (clone ABT-CK16) | IHC-P | Mouse IgG |
| VS3-CJ525 | Mouse Anti-KRT16 Recombinant Antibody (clone PT1870) | IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1211 | Anti-KRT16 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3850 | Anti-Rat KRT16 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3849 | Anti-Human KRT16 Immunohistochemistry Kit | IHC |
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