Recombinant Rabbit Anti-KRT7 Antibody (clone R03-2E7)
CAT#: VS3-FY830
This product is a recombinant rabbit antibody that recognizes KRT7. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R03-2E7 is specific for human KRT7. The antigen is synthetic peptide of human cytokeratin 7.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7315/1891_G5_16_cr5ba0bd7759116_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/1999_A12_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/170875_B_3_5_selected.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28/299_B_2_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, HBEC3-KT, hTERT-HME1, RT4, SiHa, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-KRT7 Antibody (clone R03-2E7) in WB.
Western Blot analysis of cytokeratin 7 in HeLa lysates using a cytokeratin 7 antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-KRT7 Antibody (clone R03-2E7) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using an antibody to cytokeratin 7. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human cytokeratin 7.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KRT7
- Species Reactivity
- Human
- Clone
- R03-2E7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 51 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY830. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KRT7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0301MZ | Recombinant Mouse Anti-Human Cytokeratin 7 Antibody (clone OW-TM 13/31) | IHC | Mouse antibody |
| MRO-0451-CN | Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (CBACN-188) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-0455-CN | Recombinant Mouse Anti-KRT7 Monoclonal Antibody (3-G3-D8) | WB, IHC | Mouse IgG1 |
| ZG-0607F | Mouse Anti-KRT7 Recombinant Antibody (clone OV-TL12/30) | IHC-Fr, IHC-P | Mouse IgG1 |
| ZG-0772F | Mouse Anti-KRT7 Recombinant Antibody (clone C35) | IHC-Fr, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1992 | Hi-Affi™ Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (DS1992AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1383 | AbPlus™ Anti-KRT7 Magnetic Beads (VS-0724-YC1383) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3884 | Anti-KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3885 | Anti-Mouse KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3887 | Anti-Rat KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3888 | Anti-Canine KRT7 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Inhib, Cyt
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Neut, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: Neut
Application: ELISA, Neut
Application: ELISA, Block, WB, FC, IP
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
