Recombinant Mouse Anti-KRT7 Antibody (VS4-CJ12) (CAT#: VS4-CJ12)
This mouse anti-KRT7 antibody is a product that recognizes human, mouse, rat KRT7. The immunogen of this antibody is a synthetic peptide corresponding to KRT7. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500), Immunofluorescence (1:50-1:200), Immunoprecipitation (1:200-1:2000).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7315/1891_G5_16_cr5ba0bd7759116_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7315/1891_G5_16_cr5ba0bd7759116_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/1999_A12_5_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/1999_A12_5_selected.jpg
Figure 3 IF staining of human cell line BJ
(Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/170875_B_3_5_selected.jpg
(Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/170875_B_3_5_selected.jpg
Figure 4 Liver
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_4.jpg
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_4.jpg
Figure 5 Kidney
(Cells in tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_5.jpg
(Cells in tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_5.jpg
Figure 6 Breast
(Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28/299_B_2_4.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28/299_B_2_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, HBEC3-KT, hTERT-HME1, RT4, SiHa, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, HBEC3-KT, hTERT-HME1, RT4, SiHa, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
Specifications
- Immunogen
- Synthetic Peptide
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat KRT7
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, WB, IHC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500), Immunofluorescence (1:50-1:200), Immunoprecipitation (1:200-1:2000).
Target
- Alternative Names
- K7; CK7; SCL; K2C7
- Gene ID
- 3855
- UniProt ID
- P08729
- Long Name
- Keratin 7
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Post Translation Modifications
- Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Protein Refseq
- NP_005547.3
- Function
- Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1992 | Hi-Affi™ Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (DS1992AB) | IHC-P | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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