Recombinant Rabbit Anti-LEFTY2 Antibody (clone 10G2)
CAT#: VS4-CJ17
This rabbit anti-LEFTY2 antibody (clone 10G2) is a product that recognizes human LEFTY2. The immunogen of this antibody is a synthesized peptide derived from human LEFTY2. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47883/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside the reaction centra.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25801/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56210/126863_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47883/116892_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25801/58819_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47883/116892_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47883/116892_A_5_6.jpg
Normal Tissue
Figure 8 Smooth muscle
Smooth muscle cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47883/116892_B_5_7.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25801/58819_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, NTERA-2, RPMI-8226, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143768-LEFTY2
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Specifications
- Immunogen
- A synthesized peptide derived from human LEFTY2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LEFTY2
- Species Reactivity
- Human
- Clone
- 10G2
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000).
Target
- Alternative Names
- EBAF; LEFTA; TGFB4; LEFTYA
- Gene ID
- 7044
- UniProt ID
- O00292
- Long Name
- Left-right determination factor 2
- Sequence Similarities
- Belongs to the TGF-beta family
- Cellular Localization
- Secreted
- Post Translation Modifications
- The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
- Protein Refseq
- NP_001165896.1; NP_003231.2
- Function
- Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "LEFTY2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1082CT | Recombinant Mouse anti-Human LEFTY2 Monoclonal antibody (182515) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2036 | Hi-Affi™ Recombinant Rabbit Anti-LEFTY2 Monoclonal Antibody (DS2036AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3982 | Anti-LEFTY2 Immunohistochemistry Kit | IHC |
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