Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) (CAT#: VS3-FY852)

This product is a recombinant rabbit antibody that recognizes LMNB1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R05-8D6 is specific for human, mouse, rat LMNB1. The antigen is synthetic peptide of human lamin b1.


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WB

Figure 1. Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) in WB.

Figure 1. Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) in WB.

Western Blot analysis of Lamin B1 in K562, Rat Brain, C6, 3T3, Hela lysates using Lamin B1 Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) in IHC-P.

Figure 2. Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lamin B1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.


Specifications

  • Immunogen
  • Synthetic peptide of human Lamin B1.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat LMNB1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R05-8D6
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 66 kDa; Observed MW: 66 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/5000-1/10000
    Immunohistochemistry-Paraffin: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • LMN; ADLD; LMN2; LMNB
  • Sequence Similarities
  • Belongs to the intermediate filament family.
  • Cellular Localization
  • Intermediate filament, Nucleus
  • Post Translation Modifications
  • B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Function
  • Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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