Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6)
CAT#: VS3-FY852
This product is a recombinant rabbit antibody that recognizes LMNB1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R05-8D6 is specific for human, mouse, rat LMNB1. The antigen is synthetic peptide of human lamin b1.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/950_C1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79757/1894_G3_4_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows nuclear membrane positivity in glandular and stroma cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong nuclear membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/155655_A_3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79954/171056_B_6_1_selected.jpg
Normal Tissue
Figure 7 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_B_8_5.jpg
Normal Tissue
Figure 8 Kidney
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_7_4.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113368-LMNB1
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WB
Figure 1. Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) in WB.
Western Blot analysis of Lamin B1 in K562, Rat Brain, C6, 3T3, Hela lysates using Lamin B1 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-LMNB1 Antibody (clone R05-8D6) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lamin B1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human Lamin B1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat LMNB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-8D6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 66 kDa; Observed MW: 66 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/5000-1/10000
Immunohistochemistry-Paraffin: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- LMN; ADLD; LMN2; LMNB
- Gene ID
- 4001
- UniProt ID
- P20700
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Nucleus
- Post Translation Modifications
- B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
- Protein Refseq
- NP_005564.1
- Function
- Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LMNB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2068 | Hi-Affi™ Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (DS2068AB) | WB, IHC, IP | IgG |
| MOR-0075-FY | Rabbit Anti-LMNB1 Recombinant Antibody (clone AFY0046) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ772 | Rabbit Anti-LMNB1 Recombinant Antibody (VS3-CJ772) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY834 | Recombinant Mouse Anti-LMNB1 Antibody (VS3-FY834) | WB, IP | Mouse IgG |
| VS3-FY853 | Recombinant Rabbit Anti-LMNB1 Antibody (clone R04-1F4) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC666 | Rabbit Anti-LMNB1 Recombinant Antibody (VS13-YC666) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| VS7-0425-WR302 | Mouse Anti-LMNB1 Recombinant Antibody (clone 2A11A2) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY176 | AbPlus™ Anti-LMNB1 Magnetic Beads (CBACN-342) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4053 | Anti-LMNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4054 | Anti-Mouse LMNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4055 | Anti-Rat LMNB1 Immunohistochemistry Kit | IHC |
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