Recombinant Mouse Anti-MAP2 Antibody (VS4-CJ26) (CAT#: VS4-CJ26)
This mouse anti-MAP2 antibody is a product that recognizes human, bovine, mouse, rat, porcine MAP2. The immunogen of this antibody is recombinant fragment, corresponding to amino acids 1636aa-1855aa of human MAP2 (C-terminus). The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
(Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
Figure 2 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 1636aa-1855aa of human MAP2 (C-terminus).
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human, Bovine, Mouse, Rat, Porcine MAP2
- Species Reactivity
- Human, Bovine, Mouse, Rat, Porcine
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- MAP-2A; MAP2B; MAP2C; MAP2
- Gene ID
- 4133
- UniProt ID
- P11137
- Long Name
- Microtubule associated protein 2
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Microtubule
- Post Translation Modifications
- Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity).
Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
- Protein Refseq
- NP_001034627.1; NP_002365.3; NP_114033.2
- Function
- The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Recommended Products
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1671z | Mouse Anti-MAP2 Recombinant Antibody (clone 22D3) | WB, ELISA, ICC, IHC, IP | Mouse IgG1, κ |
| MOB-1172CT | Recombinant Mouse anti-Rat MAP2 Monoclonal antibody (6G0) | IHC-P, WB | |
| VS3-QX713 | Mouse Anti-MAP2 Recombinant Antibody (clone 1D7-B9-C8-F6) | WB, IP, ICC | Mouse IgG2b |
| VS3-CJ67 | Mouse Anti-MAP2 Recombinant Antibody (VS3-CJ67) | WB, ELISA | Mouse IgG |
| VS3-XY1024 | Mouse Anti-MAP2 Recombinant Antibody (clone 5B7) | ELISA, WB, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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