Recombinant Mouse Anti-MAP2 Antibody (VS4-CJ26)
CAT#: VS4-CJ26
This mouse anti-MAP2 antibody is a product that recognizes human, bovine, mouse, rat, porcine MAP2. The immunogen of this antibody is recombinant fragment, corresponding to amino acids 1636aa-1855aa of human MAP2 (C-terminus). The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 1636aa-1855aa of human MAP2 (C-terminus).
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human, Bovine, Mouse, Rat, Porcine MAP2
- Species Reactivity
- Human, Bovine, Mouse, Rat, Porcine
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- MAP-2A; MAP2B; MAP2C; MAP2
- Gene ID
- 4133
- UniProt ID
- P11137
- Long Name
- Microtubule associated protein 2
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Microtubule
- Post Translation Modifications
- Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity).
Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
- Protein Refseq
- NP_001034627.1; NP_002365.3; NP_114033.2
- Function
- The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1172CT | Recombinant Mouse anti-Rat MAP2 Monoclonal antibody (6G0) | IHC-P, WB | |
| ZG-0267C | Mouse Anti-MAP2 Recombinant Antibody (ZG-0267C) | WB | Mouse IgG |
| ZG-0268C | Mouse Anti-MAP2 Recombinant Antibody (ZG-0268C) | WB, IF | Mouse IgG |
| ZG-0269C | Mouse Anti-MAP2 Recombinant Antibody (ZG-0269C) | WB, ICC | Mouse IgG |
| VS4-CJ25 | Recombinant Mouse Anti-MAP2 Antibody (VS4-CJ25) | ELISA, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0797MZ | Chicken Anti-MAP2 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1159MZ | Chicken Anti-Microtubule Associated Protein 2 (MAP2) Polyclonal IgY | ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2130 | Hi-Affi™ Recombinant Rabbit Anti-MAP2 Monoclonal Antibody (DS2130AB) | IHC-P, IP, WB | IgG |
| MOR-0097-FY | Rabbit Anti-MAP2 Recombinant Antibody (clone AFY0068) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY161 | CytoStream™ Mouse Anti-MAP2 Recombinant Antibody (VS-0225-XY161) | FC | Mouse IgG1, kappa |
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