Recombinant Rabbit Anti-MAP3K5 Antibody (clone R06-4F5)
CAT#: VS3-FY882
This product is a recombinant rabbit antibody that recognizes MAP3K5. This antibody has been reported for use in Western Blot. The clone R06-4F5 is specific for human MAP3K5. The antigen is synthetic peptide of human ask1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7824/624_F1_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/147158_B_9_5.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/147158_B_6_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/147158_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/147158_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/147158_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63245/147158_A_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ hTERT+)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197442-MAP3K5
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WB
Figure 1. Recombinant Rabbit Anti-MAP3K5 Antibody (clone R06-4F5) in WB.
Western Blot analysis of ASK1 in HeLa lysates using an ASK1 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human ASK1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAP3K5
- Species Reactivity
- Human
- Clone
- R06-4F5
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 155 kDa; Observed MW: 155 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Related Diseases
Endometrial Cancer
Throid Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAP3K5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0518MZ | Recombinant Mouse Anti-Human MAP3K5 Antibody | WB | Mouse antibody |
| MRO-0125-CN | Recombinant Rabbit Anti-MAP3K5 Monoclonal Antibody (CBACN-044) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1661-CN | Rabbit Anti-MAP3K5 Polyclonal Antibody (MRO-1661-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-WK1117 | Rabbit Anti-MAP3K5 Recombinant Antibody (VS3-WK1117) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR344 | Mouse Anti-MAP3K5 Recombinant Antibody (VS7-0425-WR344) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2142 | Hi-Affi™ Recombinant Rabbit Anti-MAP3K5 Monoclonal Antibody (DS2142AB) | WB, FC, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4221 | Anti-MAP3K5 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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