Recombinant Rabbit Anti-MMP3 Antibody (clone R04-8G6)
CAT#: VS3-FY940
This product is a recombinant rabbit antibody that recognizes MMP3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R04-8G6 is specific for human, mouse, rat MMP3. The antigen is human mmp3 synthetic peptide.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-MMP3 Antibody (clone R04-8G6) in WB.
Western Blot analysis of MMP3 in rat brain, C6, 3T3 lysates using MMP3 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-MMP3 Antibody (clone R04-8G6) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using an antibody to MMP3. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/39_D12_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_A_6_6.jpg
Normal Tissue
Figure 7 Endometrium
Cells in endometrial stroma Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_B_6_3.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7875/20627_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (BJ, BJ hTERT+, fHDF/TERT166)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149968-MMP3
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Specifications
- Immunogen
- Human MMP3 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat MMP3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-8G6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 54 kDa; Observed MW: 54 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
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Related Diseases
Rheumatoid Arthritis
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MMP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0007MZ | Recombinant Mouse Anti-Human MMP-3 Antibody (clone C1235M) | IHC-P, ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0716MZ | Chicken Anti-MMP3 Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2259 | Hi-Affi™ Recombinant Rabbit Anti-MMP3 Monoclonal Antibody (DS2259AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4651 | Hi-Affi™ Recombinant Rabbit Anti-MMP3 Monoclonal Antibody (TH164DS) | WB, IF, ICC, IHC-P, FC, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1013-CN | Recombinant Rabbit Anti-MMP3 Monoclonal Antibody (JM46-22) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2038-CN | Rabbit Anti-MMP3 Polyclonal Antibody (MRO-2038-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0691F | Mouse Anti-MMP3 Recombinant Antibody (clone 1B4) | WB, ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX782 | Mouse Anti-MMP3 Recombinant Antibody (clone 4F10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1356 | Anti-MMP3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4455 | Anti-Human MMP3 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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