Recombinant Mouse Anti-MPO Antibody (clone 9D10-2F3-3E7)
CAT#: VS3-FY942
This product is a recombinant mouse antibody that recognizes MPO. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 9D10-2F3-3E7 is specific for human MPO. The antigen is synthetic peptides derived from human myeloperoxidase (mpo).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
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IHC-P
Figure 1. Recombinant Mouse Anti-MPO Antibody (clone 9D10-2F3-3E7) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human spleen using myeloperoxidase antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptides derived from human myeloperoxidase (MPO).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MPO
- Species Reactivity
- Human
- Clone
- 9D10-2F3-3E7
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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Isotype Control
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Reconstitution Buffer
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Recommended Dilution Buffer
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1365 | Anti-MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4482 | Anti-Mouse MPO Immunohistochemistry Kit | IHC | |
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