Recombinant Rabbit Anti-MSH2 Antibody (clone R04-8B4)
CAT#: VS3-FY959
This product is a recombinant rabbit antibody that recognizes MSH2. This antibody has been reported for use in Western Blot. The clone R04-8B4 is specific for human, rat MSH2. The antigen is synthetic peptide of human msh2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66845/1768_C3_11_cr594bb51d24b1e_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/26473_A_9_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/26473_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/26473_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095002-MSH2
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WB
Figure 1. Recombinant Rabbit Anti-MSH2 Antibody (clone R04-8B4) in WB.
Western Blot analysis of MSH2 in rat brain, CHO-K1, Hela lysates using MSH2 Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human MSH2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat MSH2
- Species Reactivity
- Human, Rat
- Clone
- R04-8B4
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 105 kDa; Observed MW: 105 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- MutS Homolog 2; HMSH2; MutS (E. Coli) Homolog 2 (Colon Cancer, Nonpolyposis Type 1); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1; DNA Mismatch Repair Protein Msh2; MutS Protein Homolog 2
- Gene ID
- 4436
- UniProt ID
- P43246
- Sequence Similarities
- Belongs to the DNA mismatch repair MutS family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
- Protein Refseq
- NP_000242.1; NP_001245210.1
- Function
- Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262).
ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MSH2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2297 | Hi-Affi™ Recombinant Rabbit Anti-MSH2 Monoclonal Antibody (DS2297AB) | FC, ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1026-CN | Recombinant Mouse Anti-MSH2 Monoclonal Antibody (10G1) | WB, IHC | Mouse IgG2b |
| MRO-1027-CN | Recombinant Mouse Anti-MSH2 Monoclonal Antibody (10G2) | WB, IHC | Mouse IgG2b |
| MRO-1028-CN | Recombinant Mouse Anti-MSH2 Monoclonal Antibody (10G3) | WB, IHC | Mouse IgG2b |
| VS3-QX786 | Mouse Anti-MSH2 Recombinant Antibody (clone 4G5-E11-D9) | WB | Mouse IgG2b |
| VS3-QX787 | Mouse Anti-MSH2 Recombinant Antibody (clone 1184CT1.3.2) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC845 | AbPlus™ Anti-MSH2 Magnetic Beads (VS-0724-YC845) | IP, Protein Purification |
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