Recombinant Mouse Anti-MSH6 Antibody (clone 7H4-4C1-9C5)
CAT#: VS3-FY963
This product is a recombinant mouse antibody that recognizes MSH6. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 7H4-4C1-9C5 is specific for human MSH6. The antigen is synthetic peptide derived from human muts homolog 6 (msh6).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9091/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, golgi apparatus & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/290_E8_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/249_E8_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_6_6.jpg
Normal Tissue
Figure 11 Ovary
Ovarian stroma cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_4_7.jpg
Normal Tissue
Figure 12 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116062-MSH6
❮
❯
IHC-P
Figure 1. Recombinant Mouse Anti-MSH6 Antibody (clone 7H4-4C1-9C5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using MSH6 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
❮
❯
Specifications
- Immunogen
- Synthetic peptide derived from human MutS homolog 6 (MSH6).
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MSH6
- Species Reactivity
- Human
- Clone
- 7H4-4C1-9C5
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- MutS Homolog 6; G/T Mismatch-Binding Protein; MutS-Alpha 160 KDa Subunit; MutS Protein Homolog 6; GTMBP; GTBP; P160; DNA Mismatch Repair Protein Msh6
- Gene ID
- 2956
- UniProt ID
- P52701
- Sequence Similarities
- Belongs to the DNA mismatch repair MutS family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- The N-terminus is blocked.
Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
- Protein Refseq
- NP_000170.1; NP_001268421.1; NP_001268422.1
- Function
- Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY963. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MSH6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-407 | Recombinant Human Anti-Human MSH6 Antibody Fab Fragment, clone D205 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1179CT | Recombinant Mouse anti-Human MSH6 Monoclonal antibody (4B20I8) | ELISA, IHC-P, WB | |
| ZG-0402J | Mouse Anti-MSH6 Recombinant Antibody (ZG-0402J) | WB, ELISA | Mouse IgG |
| VS7-0425-WR421 | Mouse Anti-MSH6 Recombinant Antibody (clone 3E1) | WB, IHC, ICC, FC | Mouse IgG2a |
| VS7-0425-WR422 | Mouse Anti-MSH6 Recombinant Antibody (clone 5B11) | WB | Mouse IgG1 |
| VS7-0425-WR423 | Mouse Anti-MSH6 Recombinant Antibody (clone 8E3A7) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2299 | Hi-Affi™ Recombinant Rabbit Anti-MSH6 Monoclonal Antibody (DS2299AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY189 | AbPlus™ Anti-MSH6 Magnetic Beads (JA84-26) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1376 | Anti-MSH6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4525 | Anti-Mouse MSH6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4524 | Anti-Human MSH6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4527 | Anti-Rat MSH6 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, Cyt, Stim, PP, Agonist
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, SPR, Inhib, FuncS
Application: WB, ELISA, Neut, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, WB, FC, IHC, IP
Application: ELISA, Neut
Application: ELISA, FC, IHC, Neut
Application: FC, IA, IF, IP, IHC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
