Recombinant Rabbit Anti-NCF4 Antibody (clone R02-3G7) (CAT#: VS3-FY1007)
This product is a recombinant rabbit antibody that recognizes NCF4. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-3G7 is specific for human NCF4. The antigen is synthetic peptide of human p40-phox.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36156/575_G12_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36156/575_G12_1_selected.jpg
Figure 2 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57975/1676_D10_1_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57975/1676_D10_1_selected.jpg
Figure 3 Colon
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10146/25535_A_7_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10146/25535_A_7_3.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10146/25535_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10146/25535_A_7_8.jpg
Figure 5 Spleen
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10146/25535_B_8_4.jpg
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10146/25535_B_8_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (HEL, HMC-1, NB-4, REH, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100365-NCF4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100365-NCF4
Specifications
- Immunogen
- Synthetic peptide of human p40-phox.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NCF4
- Species Reactivity
- Human
- Clone
- R02-3G7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 39 kDa; Observed MW: 39 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Neutrophil Cytosolic Factor 4; Neutrophil NADPH Oxidase Factor 4; SH3 And PX Domain-Containing Protein 4; Neutrophil Cytosolic Factor 4, 40kDa; P40-Phox; P40PHOX; SH3PXD4
- Gene ID
- 4689
- UniProt ID
- Q15080
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NCF4"
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2307z | Mouse Anti-NCF4 Recombinant Antibody (clone 29H12) | WB, IHC | Mouse IgG1 |
| MOB-1139CT | Recombinant Mouse anti-Human NCF4 Monoclonal antibody (5I474) | WB |
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For Research Use Only. Not For Clinical Use.
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